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alexa fluor 488 glut1  (R&D Systems)


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    R&D Systems alexa fluor 488 glut1
    Cytoplasmic ROS inhibits IL-17A production in CD161 ++ CD8 + T cells. A Frequency of CD36 expression on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). B Mean fluorescence intensity (MFI) of <t>Glut1</t> on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). C MFI of CD98 on CD161 ++ CD8 + T cells from IR ( n = 7) and INR ( n = 7). D - E MFI of Cytoplasmic ROS (cytoROS, D ) and mitochondrial mass (MM, E ) in CD161 ++ CD8 + T cells from IR ( n = 10) and INR ( n = 10). F - G IL-17A levels of CD161 ++ CD8 + T cells from HIV-negative healthy donors, in the presence of medium or the ROS inhibitor N-Acetylcysteine (NAC, n = 8). A representative flow cytometry plot is shown in F . P values less than 0.05 are considered significant. *, P < 0.05; ns, P > 0.05
    Alexa Fluor 488 Glut1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+glut1/Human+Glut1+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pmc13112617-54-59-63
    Average 94 stars, based on 7 article reviews
    alexa fluor 488 glut1 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Excessive reactive oxygen species attenuated IL-17 production in CD161 ++ CD8 + T cell subset via repression of FOS in HIV-infected immunological non-responders"

    Article Title: Excessive reactive oxygen species attenuated IL-17 production in CD161 ++ CD8 + T cell subset via repression of FOS in HIV-infected immunological non-responders

    Journal: Cell Communication and Signaling : CCS

    doi: 10.1186/s12964-026-02792-5

    Cytoplasmic ROS inhibits IL-17A production in CD161 ++ CD8 + T cells. A Frequency of CD36 expression on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). B Mean fluorescence intensity (MFI) of Glut1 on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). C MFI of CD98 on CD161 ++ CD8 + T cells from IR ( n = 7) and INR ( n = 7). D - E MFI of Cytoplasmic ROS (cytoROS, D ) and mitochondrial mass (MM, E ) in CD161 ++ CD8 + T cells from IR ( n = 10) and INR ( n = 10). F - G IL-17A levels of CD161 ++ CD8 + T cells from HIV-negative healthy donors, in the presence of medium or the ROS inhibitor N-Acetylcysteine (NAC, n = 8). A representative flow cytometry plot is shown in F . P values less than 0.05 are considered significant. *, P < 0.05; ns, P > 0.05
    Figure Legend Snippet: Cytoplasmic ROS inhibits IL-17A production in CD161 ++ CD8 + T cells. A Frequency of CD36 expression on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). B Mean fluorescence intensity (MFI) of Glut1 on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). C MFI of CD98 on CD161 ++ CD8 + T cells from IR ( n = 7) and INR ( n = 7). D - E MFI of Cytoplasmic ROS (cytoROS, D ) and mitochondrial mass (MM, E ) in CD161 ++ CD8 + T cells from IR ( n = 10) and INR ( n = 10). F - G IL-17A levels of CD161 ++ CD8 + T cells from HIV-negative healthy donors, in the presence of medium or the ROS inhibitor N-Acetylcysteine (NAC, n = 8). A representative flow cytometry plot is shown in F . P values less than 0.05 are considered significant. *, P < 0.05; ns, P > 0.05

    Techniques Used: Expressing, Fluorescence, Flow Cytometry

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    Article Title: Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells
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    Article Title: Targeting p53 and histone methyltransferases restores exhausted CD8+ T cells in HCV infection.
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    Article Title: Targeting p53 and histone methyltransferases restores exhausted CD8+ T cells in HCV infection
    Article Snippet: Peptides covering the HLA-A2-restricted genotype-specific HCV epitopes corresponding to NS3 amino-acids 1073–1081, 1406–1415, NS4 1992–2000, and NS5 2627–2635, and to the influenza virus (FLU) matrix amino-acids 58–66 (Mimotopes, Victoria, Australia) and the corresponding PE-labeled or APC-labeled dextramer peptide-HLA class I complexes from Immudex (Copenhagen, Denmark) were used for virus-specific T cell functional assays. .. Anti-Human CD3 (BD HorizonTM PE-CF594, clone HCHT1, 1:200, Cat# 562280); Anti-Human CD3 (BD Horizon APC-R700, clone HCHT1, 1:200,Cat# 565119); Anti-Human CD3 (PE/Cy7, clone HIT3a, BioLegend, 1:100, Cat#300316); Anti-Human CD8 (PE-CyTM7, clone RPA-T8, BD Biosciences, 1:100, Cat#557746); Anti-Human CD8 (CD8-AlexaFluor®700, clone RPA-T8, BD Biosciences, 1:50, Cat#557945); Anti-Human CD8 (PerCP, clone BW135/80, Miltenyi Biotec, 1:200, Cat#130-113-160); Anti-Human CD8 (BD PharmingenTM APC-H7, clone SK1, BD Biosciences, 1:50, Cat# 560179); Anti-Human CD4, PE, clone RPA-T4, BD Biosciences, 1:50, Cat# 555347); Anti-Human CD4, AlexaFluor®647, clone RPA-T4, BD Biosciences, 1:50, Cat#557707); Anti-Human IFN-γ (APC-R700, clone B27, BD Biosciences 1:200, Cat# 564981); Anti-Human IL-2 (APC, Clone MQ1-17H12, BD Biosciences, 1:50, Cat# 554567); Anti-Human CD279 (PD-1) (PE/Cy7, clone EH12.2H7, BioLegend, 1:20, Cat# 329918); Anti-Human TNF-α (FITC, Clone cA2, Miltenyi Biotec, 1:200, Cat# 130-091-650); Anti-Human Glut1 (FITC, Clone # 202915, R&D Systems, 1:20, Cat# FAB1418F); Anti-Phosphorylated Human p38 MAPK (pT180/pY182) (PE-Cy7, Clone 36/p38, BD Biosciences, 1:20, Cat# 560241); Anti-Human p53 (FITC, Clone DO-7, BioLegend, 1:20, Cat# 645804); Anti-Human phospho-p53 (S15) (PE, Clone 261352, R&D Systems, 1:50, Cat# IC1839P); Anti-Human phospho-ATM (Ser1981) (PE, Clone 10H11.E12, BioLegend, 1:100, Cat# 651204); Anti-Human dimethyl-Histone H3 (Lys9)(AlexaFluor®488, Merck Millipore, 1:100, Cat# FCABS301A4); Anti-Human acetyl-Histone H3 (PE, Merck Millipore, 1:100, Cat# FCABS325PE); Anti-Human HLA-A2 (PE, Clone BB7.2, BioLegend, 1:200, Cat# 343306), and the viability probe 7-AAD (BD Biosciences) were used for T cell staining. .. LEAF purified anti-CD3 (clone HIT3a, BioLegend) and anti-CD28 (clone CD28.2, BioLegend) were used for overnight T cell stimulation.

    Flow Cytometry:

    Article Title: Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells
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    Article Title: Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells
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    Recombinase Polymerase Amplification:

    Article Title: Targeting p53 and histone methyltransferases restores exhausted CD8+ T cells in HCV infection.
    Article Snippet: Peptides covering the HLA-A2-restricted genotype-specific HCV epitopes corresponding to NS3 aminoacids 1073–1081, 1406–1415, NS4 1992–2000, and NS5 2627–2635, and to the influenza virus (FLU) matrix amino-acids 58–66 (Mimotopes, Victoria, Australia) and the corresponding PE-labeled or APC-labeled dextramer peptide-HLA class I complexes from Immudex (Copenhagen, Denmark) were used for virus-specific T cell functional assays. .. Anti-Human CD3 (BD HorizonTM PE-CF594, clone HCHT1, 1:200, Cat# 562280); Anti-Human CD3 (BD Horizon APC-R700, clone HCHT1, 1:200,Cat# 565119); Anti-Human CD3 (PE/Cy7, clone HIT3a, BioLegend, 1:100, Cat#300316); Anti-Human CD8 (PE-CyTM7, clone RPA-T8, BD Biosciences, 1:100, Cat#557746); Anti-Human CD8 (CD8-AlexaFluor®700, clone RPA-T8, BD Biosciences, 1:50, Cat#557945); Anti-Human CD8 (PerCP, clone BW135/80, Miltenyi Biotec, 1:200, Cat#130-113-160); Anti-Human CD8 (BD PharmingenTM APC-H7, clone SK1, BD Biosciences, 1:50, Cat# 560179); Anti-Human CD4, PE, clone RPA-T4, BD Biosciences, 1:50, Cat# 555347); Anti-Human CD4, AlexaFluor®647, clone RPAT4, BD Biosciences, 1:50, Cat#557707); Anti-Human IFN-γ (APC-R700, clone B27, BD Biosciences 1:200, Cat# 564981); Anti-Human IL-2 (APC, Clone MQ1-17H12, BD Biosciences, 1:50, Cat# 554567); Anti-Human CD279 (PD-1) (PE/Cy7, clone EH12.2H7, BioLegend, 1:20, Cat# 329918); Anti-Human TNF-α (FITC, Clone cA2, Miltenyi Biotec, 1:200, Cat# 130-091-650); Anti-Human Glut1 (FITC, Clone # 202915, R&D Systems, 1:20, Cat# FAB1418F); Anti-Phosphorylated Human p38 MAPK (pT180/pY182) (PE-Cy7, Clone 36/p38, BD Biosciences, 1:20, Cat# 560241); Anti-Human p53 (FITC, Clone DO-7, BioLegend, 1:20, Cat# 645804); Anti-Human phospho-p53 (S15) (PE, Clone 261352, R&D Systems, 1:50, Cat# IC1839P); Anti-Human phospho-ATM (Ser1981) (PE, Clone 10H11.E12, BioLegend, 1:100, Cat# 651204); Anti-Human dimethyl-Histone H3 (Lys9) (AlexaFluor®488, Merck Millipore, 1:100, Cat# FCABS301A4); Anti-Human acetyl-Histone H3 (PE, Merck Millipore, 1:100, Cat# FCABS325PE); Anti-Human HLA-A2 (PE, Clone BB7.2, BioLegend, 1:200, Cat# 343306), and the viability probe 7-AAD (BD Biosciences) were used for T cell staining. .. LEAF purified anti-CD3 (clone HIT3a, BioLegend) and anti-CD28 (clone CD28.2, BioLegend) were used for overnight T cell stimulation.

    Article Title: Targeting p53 and histone methyltransferases restores exhausted CD8+ T cells in HCV infection
    Article Snippet: Peptides covering the HLA-A2-restricted genotype-specific HCV epitopes corresponding to NS3 amino-acids 1073–1081, 1406–1415, NS4 1992–2000, and NS5 2627–2635, and to the influenza virus (FLU) matrix amino-acids 58–66 (Mimotopes, Victoria, Australia) and the corresponding PE-labeled or APC-labeled dextramer peptide-HLA class I complexes from Immudex (Copenhagen, Denmark) were used for virus-specific T cell functional assays. .. Anti-Human CD3 (BD HorizonTM PE-CF594, clone HCHT1, 1:200, Cat# 562280); Anti-Human CD3 (BD Horizon APC-R700, clone HCHT1, 1:200,Cat# 565119); Anti-Human CD3 (PE/Cy7, clone HIT3a, BioLegend, 1:100, Cat#300316); Anti-Human CD8 (PE-CyTM7, clone RPA-T8, BD Biosciences, 1:100, Cat#557746); Anti-Human CD8 (CD8-AlexaFluor®700, clone RPA-T8, BD Biosciences, 1:50, Cat#557945); Anti-Human CD8 (PerCP, clone BW135/80, Miltenyi Biotec, 1:200, Cat#130-113-160); Anti-Human CD8 (BD PharmingenTM APC-H7, clone SK1, BD Biosciences, 1:50, Cat# 560179); Anti-Human CD4, PE, clone RPA-T4, BD Biosciences, 1:50, Cat# 555347); Anti-Human CD4, AlexaFluor®647, clone RPA-T4, BD Biosciences, 1:50, Cat#557707); Anti-Human IFN-γ (APC-R700, clone B27, BD Biosciences 1:200, Cat# 564981); Anti-Human IL-2 (APC, Clone MQ1-17H12, BD Biosciences, 1:50, Cat# 554567); Anti-Human CD279 (PD-1) (PE/Cy7, clone EH12.2H7, BioLegend, 1:20, Cat# 329918); Anti-Human TNF-α (FITC, Clone cA2, Miltenyi Biotec, 1:200, Cat# 130-091-650); Anti-Human Glut1 (FITC, Clone # 202915, R&D Systems, 1:20, Cat# FAB1418F); Anti-Phosphorylated Human p38 MAPK (pT180/pY182) (PE-Cy7, Clone 36/p38, BD Biosciences, 1:20, Cat# 560241); Anti-Human p53 (FITC, Clone DO-7, BioLegend, 1:20, Cat# 645804); Anti-Human phospho-p53 (S15) (PE, Clone 261352, R&D Systems, 1:50, Cat# IC1839P); Anti-Human phospho-ATM (Ser1981) (PE, Clone 10H11.E12, BioLegend, 1:100, Cat# 651204); Anti-Human dimethyl-Histone H3 (Lys9)(AlexaFluor®488, Merck Millipore, 1:100, Cat# FCABS301A4); Anti-Human acetyl-Histone H3 (PE, Merck Millipore, 1:100, Cat# FCABS325PE); Anti-Human HLA-A2 (PE, Clone BB7.2, BioLegend, 1:200, Cat# 343306), and the viability probe 7-AAD (BD Biosciences) were used for T cell staining. .. LEAF purified anti-CD3 (clone HIT3a, BioLegend) and anti-CD28 (clone CD28.2, BioLegend) were used for overnight T cell stimulation.



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    Cytoplasmic ROS inhibits IL-17A production in CD161 ++ CD8 + T cells. A Frequency of CD36 expression on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). B Mean fluorescence intensity (MFI) of Glut1 on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). C MFI of CD98 on CD161 ++ CD8 + T cells from IR ( n = 7) and INR ( n = 7). D - E MFI of Cytoplasmic ROS (cytoROS, D ) and mitochondrial mass (MM, E ) in CD161 ++ CD8 + T cells from IR ( n = 10) and INR ( n = 10). F - G IL-17A levels of CD161 ++ CD8 + T cells from HIV-negative healthy donors, in the presence of medium or the ROS inhibitor N-Acetylcysteine (NAC, n = 8). A representative flow cytometry plot is shown in F . P values less than 0.05 are considered significant. *, P < 0.05; ns, P > 0.05

    Journal: Cell Communication and Signaling : CCS

    Article Title: Excessive reactive oxygen species attenuated IL-17 production in CD161 ++ CD8 + T cell subset via repression of FOS in HIV-infected immunological non-responders

    doi: 10.1186/s12964-026-02792-5

    Figure Lengend Snippet: Cytoplasmic ROS inhibits IL-17A production in CD161 ++ CD8 + T cells. A Frequency of CD36 expression on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). B Mean fluorescence intensity (MFI) of Glut1 on CD161 ++ CD8 + T cells from IR ( n = 13) and INR ( n = 13). C MFI of CD98 on CD161 ++ CD8 + T cells from IR ( n = 7) and INR ( n = 7). D - E MFI of Cytoplasmic ROS (cytoROS, D ) and mitochondrial mass (MM, E ) in CD161 ++ CD8 + T cells from IR ( n = 10) and INR ( n = 10). F - G IL-17A levels of CD161 ++ CD8 + T cells from HIV-negative healthy donors, in the presence of medium or the ROS inhibitor N-Acetylcysteine (NAC, n = 8). A representative flow cytometry plot is shown in F . P values less than 0.05 are considered significant. *, P < 0.05; ns, P > 0.05

    Article Snippet: For the detection of cell surface proteins, PBMCs were extracted and individually stained with the following antibodies: PE-Cy7 CD3, PerCP-Cy5.5 CD8, Alexa Fluor 488 TIM3, APC CD57, APC HLA-DR, BV421 CD38, BV510 CD98 (BD Biosciences, San Jose, CA, USA), BV421 CD36, BV421 PD-1, PE CD161 (BioLegend, San Diego, CA, USA) / FITC CD161 (BD Biosciences, San Jose, CA, USA), Alexa Fluor 488 Glut1 (R&D Systems, Minneapolis, MN, USA), FITC TIGIT (Thermo Fisher, Waltham, MA, USA).

    Techniques: Expressing, Fluorescence, Flow Cytometry